David J. Haile, M.D.

Assistant Professor, Department of Medicine

Division of Hematology

I am interested in studying iron dependent gene regulation. I am presently studying an in vitro model of the intestial epithelial cell barrier and attempting to determine the mechanisms by which iron regulated iron uptake by these cells. Ultimately, we hope to clone the iron regulated genes that are responsible for iron uptake by these cells and characterize the mechanism of iron dependent regulation.

Haile DJ, Hentze MW, Rouault TA, Harford JB and Klausner RD. (l989) Regulation of interaction of the iron-responsive element binding protein with iron-responsive RNA elements. Mol. Cell. Biol. 9, 5055-506l.

Haile DJ, Rouault TA, Harford JB and Klausner RD. (l990) The inhibition of the iron-responsive element RNA-protein interaction by heme does not mimic in vivo iron regulation. J. Biol. Chem. 265, 22:l2786-l2789.

Haile DJ, Rouault TA, Tang CK, Chin J, Harford JB and Klausner RD. (l992) Reciprocal control of RNA binding and aconitase activity in the regulation of the iron responsive element binding protein: role of the iron-sulfur cluster. Proc. Natl. Acad. Sci. USA 89, 7536-7540.

Haile DJ, Rouault TA, Harford JB, Kennedy MC, Blondin GA, Beinert H, and Klausner RD. (l992) Cellular regulation of the iron-responsive element binding protein: Disassembly of the cubane iron-sulfur cluster results in high affinity RNA binding. Proc. Natl. Acad. Sci. USA 89, ll735-ll739.

Dancis A, Yuan D, Haile DJ, Askwith C, Eide D, Moehle C, Kaplan J and Klausner RD. (l994) Molecular characterization of a copper transport protein in Saccharomyces cerevisiae: an unexpected role for copper in iron transport. Cell. 76, 393-402.

iron, iron transport, intestinal iron absorption, RNA/protein interactions